Effects of marginal dietary vitamin B3 levels on metabolic flexibility and anti-oxidant response in mice exposed to a high fat diet.

  • Maria Hegeman Wageningen University, Wageningen, the Netherlands
  • Wenbiao Shi Wageningen University, Wageningen, the Netherlands
  • Jing Tang Wageningen University, Wageningen, the Netherlands
  • Dorien van Dartel Wageningen University, Wageningen, the Netherlands
  • Hans Swarts Wageningen University, Wageningen, the Netherlands
  • Jaap Keijer Wageningen University, Wageningen, the Netherlands

Abstract

Objective: Nicotinamide adenine dinucleotide (NAD+) preserves cellular redox state and basal energy metabolism. Maintenance of NAD+ levels is dependent on dietary vitamin B3 levels, which is a NAD+ precursor. Although it is evident that severe vitamin B3 deficiency causes disease, little is known on the physiological consequences of marginal vitamin B3 levels. Therefore, the effects of marginal dietary vitamin B3 levels on metabolic flexibility and anti-oxidant response were studied.

Methods: Adult male mice were exposed to a HFD containing 5 or 30 mg nicotinamide riboside (NR) per kg diet. Body weight, lean mass, fat mass and feed intake were measured weekly. After 14 weeks, metabolic flexibility was assessed with a fasting and refeeding challenge using indirect calorimetry. After 15 weeks, the anti-oxidant response was determined in epididymal white adipose tissue.

Results: Whole body physiological parameters were not affected by dietary NR. Upon a fasting and refeeding challenge, the delta respiratory exchange ratio (Ξ”RER) was higher when comparing 30 to 5 mg/kg NR. Expression levels of anti-oxidant genes were enhanced in the 30 mg/kg group compared to the 5 mg/kg group.

Conclusion: Current results suggest that 30 mg/kg NR increases metabolic flexibility and anti-oxidant levels compared to 5 mg/kg NR.

Author Biographies

Maria Hegeman, Wageningen University, Wageningen, the Netherlands
Human and Animal Physiology
Wenbiao Shi, Wageningen University, Wageningen, the Netherlands
Human and Animal Physiology
Jing Tang, Wageningen University, Wageningen, the Netherlands
Human and Animal Physiology
Dorien van Dartel, Wageningen University, Wageningen, the Netherlands
Human and Animal Physiology
Hans Swarts, Wageningen University, Wageningen, the Netherlands
Human and Animal Physiology
Jaap Keijer, Wageningen University, Wageningen, the Netherlands
Human and Animal Physiology
Published
2016-05-10
How to Cite
HEGEMAN, Maria et al. Effects of marginal dietary vitamin B3 levels on metabolic flexibility and anti-oxidant response in mice exposed to a high fat diet.. Journal of International Society of Antioxidants in Nutrition & Health, [S.l.], v. 3, n. 3, may 2016. ISSN 2495-9405. Available at: <http://journal.isanh.net/article/view/1225>. Date accessed: 28 mar. 2017. doi: https://doi.org/10.18143/JISANH_v3i3_1225.
Section
Journal of ISANH Conference articles